By Alton Meister
Ribonuclease P: An Enzyme with a Catalytic RNA Subunit (S. Altman).
rules of Escherichia coli Glutamine Synthetase (S. Rhee, et al.).
Glucose 6-Phosphatase: techniques of Membrane-Function courting (K. Sukalski & R. Nordlie).
Chiral Phosphorothioates: Stereochemical research of Enzymatic Substitution at Phosphorus (P. Frey).
Serotonin and Peptide Immunoneuromodulators: fresh Discoveries and New principles (D. Silverman & M. Karnovsky).
The Phosphyglycerate Mutases (L. Fothergill-Gilmore & H. Watson).
Mechanism and law of the Glutamine-Dependent Carbamyl Phosphate Synthetase of Escherichia coli (A. Meister).
Cummulative Index, Vols.
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Extra resources for Advances in Enzymology and Related Areas of Molecular Biology, Volume 62
022 sec-I at 37°C. It should be pointed out that binding of either Mg(I1) or Mn(I1) to the n2 site also causes a rapid release of one equivalent of H + , but no slow H + release step was observed (57). 2) at 37°C for the nl-metalM and -5 x lop5 M for Mn(I1) and cation complex is -5 x Mg(II), respectively. Adenylylation of the enzyme causes a decrease in divalent-cation affinity at the nl site, and this effect is more pronounced for Mn(I1) binding than for Mg(I1) binding. The rate for the slow conformational change step is essentially independent of the adenylylation state of the enzyme.
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123, 127 (1986). 73. E. , Proc. Natl. Acad. Sci. USA 83, 1271(1986). 74. M. , Proc. Nail. Acad. Sci. USA 84, 7383 (1987). 75. , Maley, F. , Proc. Natl. Acad. Sci. USA 81, 3049 (1984). 76. ,Science 218, 532 (1982). Advances in Enzymology and Related Areas of Molecular Biology, Volume 62 Edited by Alton Meister Copyright © 1989 by John Wiley & Sons, Inc. REGULATION OF ESCHERICHIA GLUTAMINE SYNTHETASE COLI By SUE GOO RHEE, P. BOON CHOCK, and EARL R. STADTMAN, Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 CONTENTS I.