By Daniel L. Purich

The Advances in Enzymology and similar parts of Molecular Biology Series is likely one of the such a lot prestigious within the box, dedicated to the most recent traits in molecular biology and enzymology. every one quantity of the sequence includes contributions from top experts within the box. less than Dr. Purich's editorship, which all started with quantity seventy two, the sequence has multiplied its insurance to incorporate thematic volumes targeting particular learn components, in addition to non-thematic volumes which include chapters with a extra common allure.

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C) Lactate jonned either aerobically or anaerobically contained jixed carbon in the carboxyl group and none i n the a- and &carbons. Further study is required before the significance of these results can be evaluated fully. Probable mechanisms of the formation of lactate containing fixed carbon have already been considered in the section on bacteria (page 166). A logical objection can be raised to the scheme in Fig. 4 which assigns an essential role to a fixation reaction solely on the basis of the presence and location of fixed carbon in a compound.

This may be because other tissues have not been examined; it is known, however, that there is no h t i o n of carbon dioxide during the dissimilation of pyruvate by pigeon breast muscle (63). In'liver fixed carbon has been demonstrated in glycogen (53) and in products of pyruvate oxidation (3,78,63,50). -Attention was focussed on this fixation particularly by the experiments of Evans (79), who found that pyruvate is oxidized by pigeon liver even in the presence of malonate, and is converted to C, dicarboxylic acids, a-ketoglutaric acid and carbon dioxide.

If every molecule of propionate was formed via decarboxylation of a symmetrical dicarboxylic acid and none directly from the glycerol, the C19 concentrations in the carboxyl group of the succinic and propionic acids should be equal. 54 per cent, respectively, for the carboxyls of succinate. The implication is that none of the propionate formed in the fermentation of glycerol has arisen by direct reduction of pyruvate prior to its conversion to fumarate and back again. This argument holds whether it is accepted that succinate contains only one fixed carbon or not.

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