By Jakob von Uexküll

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10. ; Li, Z. Unfolded protein response in cancer: the physician’s perspective. J. Hematol. Oncol. 2011, 4, 8. ; ACS Symposium Series; American Chemical Society: Washington, DC, 2013. mx. Phone: +52 (81) 83284132. Fax: +52 (81) 83581400 Mammalian, microbial (bacterial and yeast), and plant cells are effective expression systems used commercially to produce mass quantities of biological, pharmaceutical, or chemical products of interest. However, there is the need to establish selective and scalable methods of product recovery that integrate effectively with upstream cell cultures to rapidly yield products in a state suitable for validation operations.

In an ATP extraction the Kp of each component can be optimized by manipulating parameters such as pH, concentration of a certain reagent, and ionic strength such that the product of interest can be forced into a phase (in this case the upper aqueous polymer phase) while the contaminants are simultaneously forced into the opposite phase (in this case the aqueous salt/buffer bottom phase, Figure 2). This type of partitioning can be seen graphically as mixture of two phases. The information in this binodal graph is critical when working with ATPS.

Benavides, J. Advances and trends in the design, analysis, and characterization of polymer-protein conjugates for “PEGylaided” bioprocesses. Anal. Bioanal. Chem. 2012, 403, 2225−35. ; Rito-Palomares, M. Current strategies and challenges for the purification of stem cells. J. Chem. Technol. Biotechnol. 2012, 87, 2–10. (a) Crawford, S. ; Labbé, M. ; Zhou, M. ; Estes, M. K. Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells. J. Virol.

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